电离辐射通过转化脂质-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘录 ：阐释伽马射线是否可通过转化酪氨酸-β（TGF-β）-激活的上皮-间质转换 (EMT)来促进骨髓的波及搬迁。使用增幅2Gy(60)Coγ线紫外线照射源自人类器官的6种骨髓，就有与EMT相关的变化，这包括分别依靠显微镜系统设计，氨基酸区别于法则，免疫荧光系统设计，划痕试验车和Transwell小室试验车来检视并检测线粒体小组织形态，EMT标记，波及搬迁能力等。转用酵素联免疫吸附法检测这些骨髓中的TGF-β蛋白水平，依靠特别胺SB431542来分析TGF-β波形通路在伽马射线EMT中的的作用。经过增幅为2Gy紫外线照射的骨髓中的长期存在间叶线粒体的表述，与假紫外线照射小组相较其上皮标记减少，间叶线粒体标记提高，同时其波及集中于能力必要性提高，TGF-β蛋白水平也提高。必要性辨认出由A549伽马射线诱导的EMT可通过对TGF-β波形抑制发生再一。这些得出结论TGF-β激活的EMT在伽马射线诱导必要性提高骨髓波及集中于能力中的起着关键作用。

校对： lizexiu